Presented at the Eighth Annual Meeting of the American Society for Cell Biology, Boston, Massachusetts, November 11-13 (1968), and Published in: J. Cell Biol. vol. 39, no. 2, part 2, p. 155a (1968):

"In Vivo Evolution within Radiation-Induced Clones of Human Lymphocytes".

Lorraine F. Meisner and John H. Frenster

Department of Medicine, Stanford University School of Medicine, Palo Alto, California

E-Mail:   frenster@euchromatin.net
Phone:   +1 650 367 6483     Fax:   +1 650 364 1773


Abstract:

Radiation of lymphocytes in vivo often results in clones of cells with stable marker chromosomes as well as unstable dicentrics, fragments and rings. Serial studies of peripheral blood lymphocytes were performed on 10 occasions over an 18 month period following 1200 rad irradiation of regional lymph nodes in a man with lymphosarcoma (1967. J. Cell Biol. 35: 91a). A minimum of 200 metaphase figures were analyzed at each of the 10 samplings. Abnormal marker chromosomes found included chiefly (1) long acrocentric chromosomes usually due to A or B chromosome inversions, (2) giant chromosomes resulting from translocations of A or B chromosomes, and (3) both single and dicentric ring chromosomes. A radiation clone was here defined by the recurrence in two or more samplings of the same association of two or more marker chromosomes within single cells. By these criteria, some 20 clones defined by double markers were identified. Three of these clones were further identified by triple markers, but no clones with higher oders of marker association were found. Each of the 60 cells noted to contain two to five marker chromosomes could be arrayed within an evolutionary map composed of primary clones, from which divergent evolution to secondary clones and single unique cells proceeded through breakage of unstable chromosome rearrangements and mitotic nondisjunction. Clones favored or disfavored by in vivo selective pressures could be identified within the evolutionary map. Ring chromosomes and pentacentric aberrations were capable of surviving in vivo for up to 18 months, and, in fact, several of the cells bearing unstable ring aberrations gave direct evidence of  in vivo cell division. Stable marker chromosomes seemed to be derived chiefly from chromosomes of groups A and B, whereas ring chromosomes seemed to arise from chromosomes in the C group.


Additional References:

1. King TJ, and Briggs R, "Serial Transplantation of Embryonic Nuclei", Cold Spring Harbor Symp. Quant. Biol. vol. 21: pp 271-290 (1956).

2. Frenster JH, Papalian MJ, Masek MA, and Frenster JA, "Electron Microscopic Analysis of Lymph Node Cellular Activity in Hodgkin's Disease", Journal of the National Cancer Institute, Vol. 63, pp. 331-335 ( Aug. 1979).


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