Published in: Cell, vol. 101, pp. 601–611, (June, 2000):
"Structural Organization of the RNA Polymerase-Promoter Open Complex".
Nikolai Naryshkin, Andrey Revyakin, Younggyu Kim, Vladimir Mekler,
and Richard H. Ebright.
Howard Hughes Medical Institute, Waksman Institute, Department of Chemistry,
Rutgers University, Piscataway, New Jersey 08854
Corresponding author: Richard H. Ebright, 732 445 5179 (phone), 732
445 5735 (fax), ebright@mbcl.rutgers.edu
Abstract:
We have used systematic site-specific protein–DNA photocrosslinking
to define interactions between bacterial RNA polymerase (RNAP) and promoter
DNA in the catalytically competent RNAP-promoter open complex (RPo).
We have mapped more than 100 distinct crosslinks between individual segments
of RNAP subunits and individual phosphates of promoter DNA. The results
provide a comprehensive description of protein–DNA interactions in RPo,
permit construction of a detailed model for the structure of RPo,
and permit analysis of effects of a transcriptional activator on the structure
of RPo.
Additional References:
"Architecture of RNA Polymerase II and Implications
for the Transcription Mechanism".
"6S RNA Regulates E. coli RNA Polymerase Activity".
Top of Page - Euchromatin
Network - Current
Research - Forums - Other
Sites - Future Events
-
For Further Information and Feedback:
E-mail: frenster@euchromatin.net
euchromatin: "the most active portion of the genome within the
cell nucleus".