Presented at the 40th Annual Meeting of the American Society for Cell Biology. San Francisco, California, December 10, 2000, and Published in Mol. Biol. Cell vol. 11, suppl. p. 20a (December, 2000):

"Nuclear Ribosomes and RNA-RNA Duplexes".

John H. Frenster

Physicians' Educational Series, 247 Stockbridge Avenue, Atherton, CA 94027-5446, USA.

E-Mail: frenster@euchromatin.net


Abstract:

Protein synthesis within the isolated calf thymus cell nucleus is DNase I-sensitive and requires a sodium rather than a potassium cation environment (Proc. Natl. Acad. Sci. USA 46: 432 (1960). Such protein synthesis occurs on nuclear ribonucleoprotein particles, in which newly-synthesized RNA is also found. After extraction from the cell nuclei, 120 S nuclear ribosomes remain active for protein synthesis in a nucleus-free system, stimulated by the addition of DNA species, but inhibited by the addition of histones or of synthetic polyanions (Biochim. Biophys. Acta  47: 130 (1961). RNA-RNA duplexes occur within the cell nucleus, involving both messenger RNA (mRNA) and pre-messenger RNA (pre-mRNA) with de-repressor RNA (Cancer Res. 36: 3394 (1976). Such RNA-RNA duplexes are involved in antisense inhibition of ribosome translation and of gene activation, and are candidates for antisense inhibition of oncogene activity (Clin. Cancer Res. 5: suppl., 3855s ( 1999). The coordinated nuclear synthesis of nuclear protein species and nuclear RNA species suggests that nuclear ribosomes may be participating in this RNA-RNA interaction. Supported in part by Research Grants CA-10174 and CA-13524 from the National Cancer Institute.


References:

1."Metabolism and Morphology of Ribonucleoprotein Particles from the Cell Nucleus of Lymphocytes".

2. "In-Vitro Incorporation of Amino Acids Into the Proteins of Isolated Nuclear Ribosomes".

3. "Selective Control of DNA Helix Openings during Gene Regulation".

4. "Oncogenes as Molecular Targets within Active Chromatin".


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