Presented at the 66th Annual Meeting of the American Association for Cancer Research, San Diego, CA, May 7, 1975, and published in: Proc. Am. Assoc. Cancer Res. 16: 10 (1975).

"Electron Microscopic Localization of Acid Phosphatase Activity within Hodgkin's Disease Lymph Nodes".

Reed A. Rowan, Marilyn A. Masek, John M. Thompson, and John H. Frenster.

Department of Medicine, Stanford University School of Medicine, Stanford, California 94305.


Abstract:

Monocytes and macrophages are increased in number within lymph nodes involved by Hodgkin's Disease, where such cells are thought to play a role in phagocytizing neoplastic cells, in re-modeling collagen, and in interacting w ith immune T-lymphocytes (Proc. Am. Assoc. Cancer Res. 14: 8 (1973).

Acid phosphatase is an important enzyme of monocytes and macrophages, and therefore the distribution of its activity within the involved lymph nodes of untreated patients with Hodgkin's Disease was determined by high-resolution electron microscopic histochemistry.

Acid phosphatase activity within lymphocytes and neoplastic Hodgkin and Reed-Sternberg cells was confined to the Golgi apparatus and the rare lysosomes, while monocytes displayed additional activity within endocytic and cytoplasmic vesicles. By contrast, macrophages displayed not only these patterns of activity, but demonstrated significant activity in apposition to neoplastic cells and within phagosomes containing collagen or lymphocytes in various stages of intracellular digestion.

These ultrastructural enzyme histochemical data suggest that monocytes and macrophages are important and possibly ambivalent elements in a T-lymphocyte immune response to neoplastic B-cells (Nature 243: 188 (1973); Lancet 2: 167 (1974) in untreated patients with Hodgkin's Disease (Natl. Cancer Inst. Monogr. 36: 239 (1973).


Additional References:

1. Chen JJW, Lin Y-C, Yao P-L, Yuan A, Chen H-Y, Shun C-T, Tsai M-F, Chen C-H, Yang P-C, "Tumor-Associated Macrophages: The Double-Edged Sword in Cancer Progression", Journal of Clinical Oncology, vol. 23, no. 5, pp. 953-964 (February 10, 2005).

2. Archibald RB, and Frenster JH, "Quantitative Ultrastructural Analysis of In-Vivo Lymphocyte - Reed-Sternberg Cell Interactions in Hodgkin's Disease", Natl. Cancer Inst. Monogr. 36: 239-245 (1973).

3. Masek MA, Rhoades DJ, and Frenster JH, "In-Vivo Macrophage Interactions with Lymphocytes in Hodgkin's Disease", Proc. Am. Assoc. Cancer Res. 14: 8 (1973).

4. Frenster JH, "Phytohemagglutinin-Activated Autochthonous Lymphocytes for Systemic Immunotherapy of Human Neoplasms".

5. Frenster JH, Papalian MM, Masek MA, and Frenster JA, "Electron Microscopic Analysis of Lymph Node Cellular Activity in Hodgkin's Disease".


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