Published in: Nature, vol. 407, no. 6802, pp. 319-320 (September 21, 2000):

"Gene Expression: Total Silencing by Intron-Spliced Hairpin RNAs".

Neil A. Smith, Surinder P. Singh, Ming-Bo Wang, Peter A. Stoutjesdijk, Allan G. Green & Peter M. Waterhouse


Abstract:

Post-transcriptional gene silencing (PTGS), a sequence-specific RNA degradation mechanism inherent in many life-forms, can be induced in plants by transforming them with either antisense or co-suppression constructs, but typically this results in only a small proportion of silenced individuals. Here we show that gene constructs encoding intron-spliced RNA with a hairpin structure can induce PTGS with almost 100% efficiency when directed against viruses or endogenous genes. These constructs could prove valuable in reverse genetics, genomics, engineering of metabolic pathways and protection against pathogens.


Additional References:

1. "Nuclear Polyanions as De-Repressors of Synthesis of Ribonucleic Acid".

2. "Mated Models of Gene Regulation in Eukaryocytes".

3. "Oncogenes as Molecular Targets within Active Chromatin".

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